Abstract
KB cells infected with dengue type 2 virus synthesize three types of RNA with sedimentation coefficients greater than 18 S. The first is soluble in 2 M LiCl, sediments as a discrete species at about 20 S, and is greater than 95% RNase resistant. The second is a discrete 40 S species, insoluble in 2 M LiCl, and more than 95% sensitive to digestion by RNase. The third, also insoluble in 2 M LiCl, is “heterogeneous” in size, sedimenting between 20 and 28 S, and is 50–70% RNase resistant. Pulse-chase studies demonstrate the “heterogeneous” and 20 S RNAs to be intermediate in the synthesis of the virus 40 S genomic RNA. The data from agarose-formaldehyde gel analysis of these RNAs are in agreement with our identification of the “heterogeneous” and 20 S RNAs as the replicative intermediate (RI) and replicative form (RF) RNAs, respectively.
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