Abstract
In model plants, such as Arabidopsis thaliana, changes in floral morphology, as well as in plant architecture, depend on the behavior of the stem meristem (vegetative, inflorescence and floral), whose control is directly related to the expression of the WUSCHEL (WUS) gene belonging to the WUSCHEL-related homeobox (WOX) gene group. The sequencing of Passiflora organensis genome will allow the identification and analysis of key genes expression in the development of this genus plants. The present work intends to identify and characterize the members of the WOX gene family in Passiflora, with a focus on investigating the role of the WUS gene in P. organensis by analyzing the gene expression by qRT-PCR and in situ hybridization.
Highlights
The superfamily of homeotic gene is characterized by a sequence called homeobox
The WUSCHEL (WUS) homeobox is a member of the WUSCHEL-related homeobox (WOX) family gene and it is expressed in the organizingcenter cells of the shoot apical meristem (SAM) and axillary meristem, and operates to regulate and garantee the stem cell maintenance[2]
Using the genomic sequence of P. organensis obtained by our research group as source for the identification of orthologs WOX genes, comparing to published sequences of A. thaliana using the BLAST algorithm
Summary
Introduction The superfamily of homeotic gene is characterized by a sequence called homeobox. These transcription factors are intimately related to organism development. In Arabidopsis thaliana, 15 homeobox genes, named WUSCHEL-related homeobox (WOX), were identified and have a wide variety of roles during the plant development[1]. The WUSCHEL (WUS) homeobox is a member of the WOX family gene and it is expressed in the organizingcenter cells of the shoot apical meristem (SAM) and axillary meristem, and operates to regulate and garantee the stem cell maintenance[2].
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