Abstract
SummaryDuring the study of fungal trunk pathogens associated with urban trees decline in Shiraz (Iran), a serious decline of willow and poplar trees was observed. Therefore, an investigation was conducted on these trees in some areas of this city during spring and summer 2012 and 2013, to determine the main fungal trunk pathogens associated with these ornamental plants. Plant materials were collected from trees exhibiting disease symptoms such as yellowing, shoot canker, shoot dieback, defoliation and internal wood necrosis and decayed wood. Fungal isolations were made from discoloured or decayed wood tissue onto 2% malt extract agar (MEA) amended with streptomycin sulphate. Nine species,Fomes fomentarius,Diplodia seriata,Lasiodiplodia theobromae,Dothiorella sarmentorum,Neoscytalidium hyalinum,Diatrype whitmanensis,Phaeoacremonium rubrigenum,P. aleophilumandP. parasiticum, were identified based on morphology and DNA sequence comparisons. Pathogenicity tests were performed on detached shoots of willow and poplar trees under greenhouse conditions.Lasiodiplodia theobromaecaused the longest lesions on willow. On poplar shoots, the longest lesions were caused byP. parasiticum.Diplodia seriataproduced the smallest lesions on both woody hosts. First reports from willow wood includeP. parasiticum, P. rubrigenum, D. whitmanensis, L. theobromae, D. seriataandN. hyalinum,while new reports from poplar wood includeP. parasiticumandDo. sarmentorum. Based on our knowledge, this is also the first report ofD. whitmanensisin Iran.
Published Version
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