Identification and characterization of Burkholderia multivorans CCA53

Hironaga Akita,Mohd Zulkhairi Mohd Yusoff,Tamotsu Hoshino,Nobutaka Nakashima,Zen-Ichiro Kimura

doi:10.1186/s13104-017-2565-1

Abstract

ObjectiveA lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53.ResultsMultilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences was performed to identify Burkholderia sp. CCA53. The MLST analysis revealed that Burkholderia sp. CCA53 was tightly clustered with B. multivorans ATCC BAA-247T. The quinone and cellular fatty acid profiles, carbon source utilization, growth temperature and pH were consistent with the characteristics of B. multivorans species. Burkholderia sp. CCA53 was therefore identified as B. multivorans CCA53.

Highlights

The genus Burkholderia was firstly proposed by Yabuuchi et al [1], and was classified as Gram-negative and nonspore forming β-proteobacteria
CCA53 was identified as B. multivorans CCA53
CCA53, we performed an Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences (Fig. 1)

Summary

Results

Using MLST analysis with housekeeping genes, several Burkholderia species were identified. The existence of Burkholderia cepacia complex species in moso bamboo plantations [26] and water bodies [6] were determined by MLST analysis based on fragments of the atpD, gltBD, gyrB, lepA, recA, phaC and trpB gene sequences. CCA53, we performed an MLST analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences (Fig. 1). No growth occurred on gelatin, glycogen, starch, inulin, d-melezitose, d-raffinose, arbutin, esculin ferric citrate, gentiobiose, d-melibiose, Fig. 1 Phylogenetic tree reconstructed from analysis of the sequences of six housekeeping genes (atpD, gltD, gyrB, lepA, recA and trpB) and show‐ ing the relationship of CCA53 with related species. These characteristics were nearly the same as those of B. multivorans NKI379, which was isolated from soil samples in the Er-Ren River Basin, Taiwan [33]

Introduction

Main text

Discussion

Limitations