Abstract
ABSTRACTTo better understand the mechanisms of plant abiotic stress responses and identify novel stress-related genes in rice, we performed global expression analysis in indica rice Pei'ai 64S under multiple stresses. Among numerous genes, a gene named OsTHIC was selected, which was highly induced in leaves and panicles in response to all stresses at different developmental stages, especially at the booting stage under cold stress. OsTHIC contains an open reading frame (ORF) of 1920 bp and encodes a predicted protein with 639 amino acid residues. The molecular weight and isoelectric point were predicted to be about 71.31 kD and 6.41, respectively. The results of quantitative real-time polymerase chain reaction (qRT-PCR) analysis were almost identical to those from the GeneChip Rice Genome Array. The sequence alignment showed about 99% similarity to rice phosphate methylpyrimidine synthase. Promoter sequence analysis showed that various stress response-related cis-elements were contained in the promoter region. All the results suggested that OsTHIC may be involved in rice stress responses.
Highlights
Abiotic stresses, such as heat, cold and drought, have severe impacts on plant growth, development and productivity [1,2,3]
All the results suggested that OsTHIC may be involved in rice stress responses
The other gene expression data are available at a public microarray database, the National Center for Biotechnology Information (NCBI) gene expression omnibus (GEO; http://www.ncbi.nlm. nih.gov/geo/) [35]: the comparisons of tissues versus untreated control (NCBI GEO accession no.GSE6893), abiotic stresses versus untreated control (GSE6901), trans-zeatin versus mock dimethyl sulfoxide in root and leaves at 30 and 120 min after treatment (GSE6737; Figure 2) [36]
Summary
Abiotic stresses, such as heat, cold and drought, have severe impacts on plant growth, development and productivity [1,2,3]. Exposure to extreme environmental conditions will lead to a dramatic decline in yield or even plant death [5]. To survive under these environmental stresses, plants have evolved the ability to respond to various abiotic stresses [6]. The molecular and cellular mechanisms underlying the adaptation of higher plants to environmental stresses have attracted more attention [7,8,9]. To improve rice abiotic stress resistance, we need to understand the mechanisms by which plants respond to these abiotic stresses
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