Abstract
BackgroundThe Pacific abalone, Haliotis discus hannai, is the most important cultivated abalone in China. Improving abalone muscle growth and increasing the rate of growth are important genetic improvement programs in this industry. MicroRNAs are important small noncoding RNA molecules that regulate post-transcription gene expression. However, no miRNAs have been reported to regulate muscle growth in H. discus hannai.Resultswe profiled six small RNA libraries for three large abalone individuals (L_HD group) and three small individuals (S_HD group) using RNA sequencing technology. A total of 205 miRNAs, including 200 novel and 5 known miRNAs, were identified. In the L_HD group, 3 miRNAs were up-regulated and 7 were down-regulated compared to the S_HD specimens. Bioinformatics analysis of miRNA target genes revealed that miRNAs participated in the regulation of cellular metabolic processes, the regulation of biological processes, the Wnt signaling pathway, ECM-receptor interaction, and the MAPK signaling pathway, which are associated with regulating growth. Bone morphogenetic protein 7 (BMP7) was verified as a target gene of hdh-miR-1984 by a luciferase reporter assay and we examined the expression pattern in different developmental stages.ConclusionThis is the first study to demonstrate that miRNAs are related to the muscle growth of H. discus hannai. This information could be used to study the mechanisms of abalone muscle growth. These DE-miRNAs may be useful as molecular markers for functional genomics and breeding research in abalone and closely related species.
Highlights
The Pacific abalone, Haliotis discus hannai, is the most important cultivated abalone in China
We investigated the miRNA profiles of H. discus hannai muscle using an Illumina HiSeq 2500 platform
To identify the known and novel miRNAs in H. discus hannai, small RNA sequences were mapped to the known mature Haliotis rufescens miRNAs from the miRBase database
Summary
The Pacific abalone, Haliotis discus hannai, is the most important cultivated abalone in China. Improving abalone muscle growth and increasing the rate of growth are important genetic improvement programs in this industry. MicroRNAs are important small noncoding RNA molecules that regulate post-transcription gene expression. No miRNAs have been reported to regulate muscle growth in H. discus hannai. The Pacific abalone, Haliotis discus hannai, is the most important cultivated abalone in China [1, 2]. Improving abalone muscle growth and increasing the rate of growth are important genetic improvement programs [3]. A better understanding of the molecular mechanisms of muscle growth can provide useful knowledge for programs that aim to improve abalone musculature. MicroRNAs (miRNAs) are small noncoding RNA molecules (18–22 nt) which regulate post-transcription gene expression by mapping target mRNA 3′ untranslated regions (UTRs) [4, 5].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
