Abstract
A new insertion sequence (IS 1383) was identified on plasmids from Pseudomonas putida strain H and its nucleotide sequence was determined. IS 1383 contains perfect terminal inverted repeats of 13-bp flanking a 1.4-kb internal sequence. A single significant open reading frame was identified that can encode a 342-amino acid polypeptide which was predicted to be highly basic and to have homology to polypeptides known from several other bacterial insertion sequences. At least six copies of IS 1383 are present on the plasmids pPGH1 and pPGH2, whereas no copy could be detected on the chromosome of P. putida strain H. Target duplications did not flank the inverted repeats of any of the six IS 1383 copies examined. Analysis of the integration sites of IS 1383 revealed hints for a target specificity. Multiple sequence alignments of the transposases, the inverted repeats and the integration sites pointed to the assignment of IS 1383 into a putative new family of insertion sequences defined as the IS 1111 family.
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