Abstract
BackgroundThe aim of this study is to screen single nucleotide polymorphisms (SNP) of chicken Calpain3 (CAPN3) gene and to analyze the potential association between CAPN3 gene polymorphisms and carcass traits in chickens. We screened CAPN3 single nucleotide polymorphisms (SNP) in 307 meat-type quality chicken from 5 commercial pure lines (S01, S02, S03, S05, and D99) and 4 native breeds from Guangdong Province (Huiyang Huxu chicken and Qingyuan Ma chicken) and Sichuan Province (Caoke chicken and Shandi Black-bone chicken), China.ResultsTwo SNPs (11818T>A and 12814T>G) were detected by single strand conformation polymorphism (SSCP) method and were verified by DNA sequencing. Association analysis showed that the 12814T>G genotypes were significantly associated with body weight (BW), carcass weight (CW), breast muscle weight (BMW), and leg muscle weight (LMW). Haplotypes constructed on the two SNPs (H1, TG; H2, TT; H3, AG; and H4, AT) were associated with BW, CW (P < 0.05), eviscerated percentage (EP), semi-eviscerated percentage (SEP), breast muscle percentage (BMP), and leg muscle percentage (LMP) (P < 0.01). Diplotype H1H2 was dominant for BW, CW, and LMP, and H2H2 was dominant for EP, SEP, and BMP.ConclusionWe speculated that the CAPN3 gene was a major gene affecting chicken muscle growth and carcass traits or it was linked with the major gene(s). Diplotypes H1H2 and H2H2 might be advantageous for carcass traits.
Highlights
The aim of this study is to screen single nucleotide polymorphisms (SNP) of chicken Calpain3 (CAPN3) gene and to analyze the potential association between CAPN3 gene polymorphisms and carcass traits in chickens
CAPN3 was identified by positional cloning as the gene responsible for limb-girdle muscular dystrophy 2A (LGMD2A) [7,8], where it plays an important role in myofibrillar integrity [9]
A PCRbased single strand conformation polymorphism (SSCP) method was successfully developed for screening the nucleotide substitutions in these exons
Summary
The aim of this study is to screen single nucleotide polymorphisms (SNP) of chicken Calpain (CAPN3) gene and to analyze the potential association between CAPN3 gene polymorphisms and carcass traits in chickens. Studies on the rate of muscle growth in layer and broiler chickens have shown that the rate of muscle growth are mainly determined by changes in the rate of muscle protein degradation, with little or no change in the rate of muscle protein synthesis [1]. I.e. intracellular Ca2+-dependent cysteine proteases (EC 3.4.22.17), have long been demonstrated to be involved in muscle growth and development and are proenzymes that are regulated by Ca2+ binding and autoproteolytic modification [2]. Study have further showed that there was less calpain activity, but more calpastatin, in broiler breast muscle [5]. Much of the available information about this gene was taken from studies on humans, mice, and other mammalian species, and might not be directly applicable to poultry
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