Abstract
This study aimed to explore the application of surface-enhanced Raman scattering (SERS) in the rapid diagnosis of gastric cancer. The SERS spectra of 68 serum samples from gastric cancer patients and healthy volunteers were acquired. The characteristic ratio method (CRM) and principal component analysis (PCA) were used to differentiate gastric cancer serum from normal serum. Compared with healthy volunteers, the serum SERS intensity of gastric cancer patients was relatively high at 722[Formula: see text]cm[Formula: see text], while it was relatively low at 588, 644, 861, 1008, 1235, 1397, 1445 and 1586[Formula: see text]cm[Formula: see text]. These results indicated that the relative content of nucleic acids in the serum of gastric cancer patients rises while the relative content of amino acids and carbohydrates decreases. In PCA, the sensitivity and specificity of discriminating gastric cancer were 94.1% and 94.1%, respectively, with the accuracy of 94.1%. Based on the intensity ratios of four characteristic peaks at 722, 861, 1008 and 1397[Formula: see text]cm[Formula: see text], CRM presented the diagnostic sensitivity and specificity of 100% and 97.4%, respectively, and the accuracy of 98.5%. Therefore, the three peak intensity ratios of I[Formula: see text]/I[Formula: see text], I[Formula: see text]/I[Formula: see text] and I[Formula: see text]/I[Formula: see text] can be considered as biological fingerprint information for gastric cancer diagnosis and can rapidly and directly reflect the physiological and pathological changes associated with gastric cancer development. This study provides an important basis and standards for the early diagnosis of gastric cancer.
Highlights
Gastric cancer is currently the fourth most common malignancy and the second leading cause of cancerrelated deaths worldwide
The results proved that the healthy volunteers and the gastric cancer patients can be di®erentiated by the Surface-enhanced Raman scattering (SERS) spectra of human plasma.[23]
It can be seen that no obvious Raman signal is detected in both normal and gastric cancer serum without using gold nanoparticles
Summary
Gastric cancer is currently the fourth most common malignancy and the second leading cause of cancerrelated deaths worldwide. Improving the diagnosis of gastric cancer is the most important approach to reducing the mortality rate of this malignancy.[1,2] Endoscopy combined with histopathological analysis is the most accurate method for the diagnosis of gastric cancer. The subjectivity of pathological diagnosis and the uncertainty of endoscopic positioning are unavoidable and often result in the misdiagnosis of small lesions in the early stage of gastric cancer.[3,4,5] identifying a highly sensitive, rapid, and noninvasive screening method for the diagnosis of gastric cancer is vital.[6,7,8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
