Abstract

Objective: Addition of proteins to hypoosmotic media delays the swelling response as compared to hypoosmotic solutions without protein supplementation. The hypoosmotically-reactive spermatozoa are able to maintain motility, and even progressive motility without associated tail swelling, in the presence of proteins in solution. The aim of this study was to assess the sperm response at the onset of swelling, and also during the development of the tail swelling patterns until reaching their optimal response under hypoosmotic conditions regulated by the presence of proteins.

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