Abstract

Two rabbits were hyperimmunisated with an ultrasound lysate of a pig origin B. bronchiseptica and one rabbit was hyperimmunisated with an ultras ound lysate of B. bronchiseptica dog strain origin. Each rabbit was inoculated three times. Blo od samples were collected before the hyperimmunisation begun and after each inoculation. Antibody producti on during immunization was controlled using the method of double diffusion in agar gel. After the first inocu lation only 2 of 3 rabbits responded with antibody production detectable with this method, but after the third in oculation all the rabbits responded with antibody p roduction at least against three antigenic compounds of the ultr asound disintegrated B.bronchiseptica . Serum samples obtained from each rabbit after the third antigen i noculation were tested against both antigen solutio ns. These tests proved that all rabbits produced antibodies a gainst the same three antigenic compounds of B. bronchiseptica.

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