Hypergravity inhibits elongation growth of azuki bean epicotyls independently of the direction of stimuli

Abstract

We examined the effects of basipetal, horizontal, and acropetal hypergravity stimulation on growth and cell wall properties of azuki bean seedlings. Horizontal and acropetal hypergravity inhibited elongation growth of epicotyls by decreasing the cell wall extensibility, as did basipetal hypergravity. Hypergravity stimulation increased the thickness of cell walls and suppressed xyloglucan breakdown regardless of direction. All hypergravity treatments increased the pH in the apoplastic fluid, which is involved in the processes of the suppression of xyloglucan breakdown. Gadolinium and lanthanum, both blockers of mechanoreceptors, nullified the growth-inhibiting effects of hypergravity. These results show that growth inhibition by hypergravity is independent of its direction in azuki bean epicotyls. The findings also suggest that mechanoreceptors on the plasma membrane perceive the gravity signal independently of its direction, and affect growth of azuki bean epicotyls.