Hymenobacter ruber sp. nov., isolated from grass soil

Abstract

A taxonomic study using a polyphasic approach was performed on a Gram-stain-negative, aerobic, non-motile, non-spore-forming, rod-shaped bacterium, designated strain PB156(T), isolated from grass soil. Comparative 16S rRNA gene sequence studies showed that the isolate was clearly affiliated with the phylum Bacteroidetes, and most closely related to Hymenobacter soli PB17(T), Hymenobacter antarcticus VUG-A42aa(T) and Hymenobacter glaciei VUG-A130(T), showing 96.4, 96.2 and 95.9 % 16S rRNA gene sequence similarity, respectively, while all other species of the genus shared only 89.3-95.2 % similarity. The main polyamine present was sym-homospermidine. The predominant menaquinone was MK-7. The major fatty acids were C15 : 0 iso, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c/t), C16 : 1ω5c and C15 : 0 anteiso. The G+C content of the genomic DNA of strain PB156(T) was 61.7 mol%. The combined genotypic and phenotypic data supported the conclusion that strain PB156(T) represents a novel species of the genus Hymenobacter, for which the name Hymenobacter ruber sp. nov. is proposed. The type strain is PB156(T) ( = KCTC 32477(T) = JCM 19433(T)).