Abstract
Ten-eleven translocation (TET) proteins are abnormally expressed in various cancers. Osteosarcoma cells were treated with hydroxyurea to investigate the expression pattern of TET proteins in these cells. The expression of TET1 was increased in U2OS cells after treatment with hydroxyurea. In addition, hydroxyurea increased cell apoptosis and altered the cell cycle. TET proteins catalyze the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC); therefore, 5mC and 5hmC levels were evaluated. Increased 5hmC levels were observed after the hydroxyurea treatment. Experiments examining cell apoptosis and the cell cycle after knockdown and overexpression of TET1 were conducted to further investigate whether TET1 expression affected cell growth. The overexpression of TET1 increased cell apoptosis and inhibited cell growth. Taken together, TET1 expression regulated proliferation and apoptosis in U2OS cells, changes that were associated with 5hmC levels.
Highlights
Osteosarcoma (OS) is the most common type of bone cancer in both adolescents and young adults [1]
The expression of TET1, TET2, and TET3 was studied in hFoB and U2OS cells using Quantitative real-time PCR (qPCR)
TET1 expression was reduced and TET2 and TET3 expression were increased in U2OS cells (Figure 1A)
Summary
Osteosarcoma (OS) is the most common type of bone cancer in both adolescents and young adults [1]. Abnormal epigenetic modifications, such as DNA methylation, have been observed in OS cells [2,3]. TET3 was proposed to function as an hsa-miR-150 target and to play a role in chronic myelomonocytic leukemia (CML) [7]. Based on these results, TET proteins are closely associated with cancer development
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