Hydrothermally Synthesized Boletus Brucella-derived Carbon Quantum Dots as a Fluorescent Probe for the Detection of Vitamin B2

Abstract

Background:: In the paper, Boletus Brucella was used as carbon source material to prepare carbon dots (CDs) by one-step hydrothermal method. The CDs had high quantum yield and high photostability. Methods:: A range of characterization studies were conducted on CDs, and the results showed that the average particle size of CDs was 5 nm, emitting blue fluorescence. The optimal excitation wavelength was 337 nm, and the emission wavelength was 440 nm. Results:: Based on the static quenching, the fluorescence of CDs could be effectively quenched by VB2. Therefore, a highly sensitive and selective fluorescent probe for detecting VB2 was constructed. The CDs were successfully used to detect tablets, human blood, and urine. Conclusion:: The recovery rate of VB2 was 97.55~99.45%, and the relative standard deviation was 1.29~3.76 (n=3). conclusion: The recovery rate of VB2 was 97.55~99.45%, and the relative standard deviation was 1.29~3.76 (n=3).