Abstract
Lead tolerance and accumulation in five willow clones were investigated using a nutrient film technique. Plants were exposed to 0, 48, 121, 169, or 241 μM Pb for 14 days. Tolerance indices (TI) and critical toxicity thresholds (EC50) were determined for five willow clones. SX61 had the highest TI values (92%) in the 48 and 121 μM Pb treatments, as well as the highest EC50 threshold values (70.5μM for roots, 155.9μM for aboveground tissue), indications of a high degree of tolerance to Pb. This clone also developed the highest biomass of all the clones tested. We found significant variation in willows' lead accumulation. The highest Pb content in roots (24 mg plant−1) and aboveground tissue (7.6 mg plant−1) was recorded in the 48 μM Pb treatment in SX61. Based on high biomass, TI, EC50, and Pb content in plant tissues, SX61 holds promise for phytoextraction of lead.
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