Hydroponic Salinity Screening by Deep Flow Technique on All Paddy Growing Phases

N Nasaruddin,Muhammad Fuad Anshori,Hari Iswoyo,Yunus Musa,Muh Arifuddin,Andi Isti Sakinah,Muh Farid Bdr

doi:10.31327/atj.v6i2.1591

Abstract

Salinity screening under hydroponic Deep Flow Technique (DFT) has not been widely studied, especially on the nature of rice tolerance to salinity stress. According to previous screening studies, this method was effective in distinguishing the nature of rice tolerance to salinity stress. However, they were tested only at generative phase. Therefore, evaluation on screening method with hydroponic DFT at all phases of paddy growth is essential. The objective of this study is to evaluate the filtering under hydroponic DFT at all paddy phase and to determine secondary character that support productivity which can be utilized as selection character in this screening process. The experiment was arranged with a complete nested group design with nested replication is the NaCl stress. There were 5 (Five) tested rice varieties and the stress environment consisted of three levels: 0, 60, and 120 mM of NaCl, all with 3 (three) replications. The nutrient culture screening was adapted to the modified Egdane method. The results showed that screening under hydroponic DFT was effective at the concentration of 60 nM of NaCl. The best selection character was yields per clump, number of productive tillers and total chlorophyll. The variety of Jeliteng, Ciherang and Inpari 34 of Salin Agritan, were classified as tolerant group. This hydroponic DFT Filtering method could be recommended as one salinity screening method for all paddy growing phases

Highlights

According to Ismail et al (2013) and Kranto S, Chankaew S, Monkham T, Theerakulpisut P, Sanitchon J. (2016) there was no significant correlations between the properties of salinity density tolerance in the vegetative and reproductive phases
It is an indication that the screening for salinity tolerance on rice by hydroponic method is possible until its reproductive phase
One hydroponic method that is practicable for this screening is the Deep Flow Technique (DFT)

Summary

Introduction

The seedlings on the rockwool were placed in a netpot container by ensuring the seedling’s root are in contact with the nutrient culture. The nutrient culture media was AB mix with a concentration of 5 ml per liter of water. The volume of nutrients in each DFT installation was 120 L. Induction of salinity stress was applied at the age of 7 days in the DFT. Stress induction was applied gradually to avoid osmotic shock. The first application of NaCl was 50% of the prescribed concentration, it is increased to 60 mM and 120 mM (as the treatments) in the following three days. The stress condition was sustained for 14 days and transferred to normal environmental conditions. The nutrient solution was replaced after 7 days under normal conditions. Administration of HCL or NaOH was to maintain the pH of the solution in the range of 5.5-6.5

Objectives

Findings

Methods