Hydrogen Sulfide Impairs Meiosis Resumption in Xenopuslaevis Oocytes.

Armance Gelaude,Jaroslav Petr,Jean-François Bodart,Matthieu Marin,Alain Martoriati,Arlette Lescuyer-Rousseau,Marketa Sedmikova,Sylvain Slaby,Caroline Molinaro,Katia Cailliau,Jan Nevoral,Veronica Kučerová-Chrpová

doi:10.3390/cells9010237

Abstract

The role of hydrogen sulfide (H2S) is addressed in Xenopus laevis oocytes. Three enzymes involved in H2S metabolism, cystathionine β-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an acceleration of oocyte meiosis resumption when inhibited. Moreover, meiosis resumption is associated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not induce the dissociation of the components of the M-phase promoting factor (MPF), cyclin B and Cdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.

Highlights

Hydrogen sulfide (H2 S) is a gasotransmitter involved in different physiological and pathological processes, including inflammation, neuromodulation, regulation of vascular remodeling, protection against heart diseases, atherosclerosis, and pulmonary hypertension [1,2]
We evaluate the impact of the gasotransmitter H2 S on the regulation of the meiosis progression in Xenopus laevis oocytes
Even at the lowest concentrations, NaHS led to a significant decrease in oocytes resuming meiosis, as attested by the decrease of white spot occurrence rates (Figure 1A)

Summary

Introduction

Hydrogen sulfide (H2 S) is a gasotransmitter involved in different physiological and pathological processes, including inflammation, neuromodulation, regulation of vascular remodeling, protection against heart diseases, atherosclerosis, and pulmonary hypertension [1,2]. CBS, CSE, and MPST are noticeably expressed in the reproductive organs of mice [6], rats [7], and humans [8]. Distinct distribution of CSE and CBS was reported in rat testis, where CSE was detected in Sertoli cells. Cells 2020, 9, 237 and CBS in Sertoli, Leydig, and germ cells, suggesting a testis function [7,11]. H2 S was mainly involved in male erectile function in primates [12], rats [13], rabbits [14], and humans [15]. A role of H2 S at the physiological level in the female reproductive system exists. CSE and CBS play different roles [17]

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Results

Conclusion