Abstract
Hydrogen gas is neuroprotective in cerebral ischemia animal models. In this study, we tested the neuroprotective effects of hydrogen saline, which is safe and easy to use clinically, in a rat model of middle cerebral artery occlusion (MCAO). Sprague-Dawley male rats weighting 250-280 g were divided into sham, MCAO plus hydrogen saline and MCAO groups, and subjected to 90-min ischemia followed by 24 h of reperfusion. Hydrogen saline was injected intraperitoneally at 1 ml/100 g body weight. Infarct volume and brain water content were evaluated at different time points after reperfusion. Oxidative stress, inflammation, and apoptotic cell death markers were measured. Hydrogen saline significantly reduced the infarct volume and edema and improved the neurological function, when it was administered at 0, 3 and 6 h after reperfusion. Hydrogen saline decreased 8-hydroxyl-2'-deoxyguanosine (8-OHdG), reduced malondidehyde, interleukin-1β, tumor necrosis factor-α, and suppressed caspase 3 activity in the ischemic brain. These findings demonstrated hydrogen saline is neuroprotective when administered within 6 h after ischemia. Because hydrogen saline is safe and easy to use, it has clinical potentials to reduce neurological injuries.
Highlights
Stroke is the second most frequent cause of death worldwide and the most frequent cause of permanent disability [1,2]
Considering the safety and the convenience, hydrogen saline has been prepared in our department and our previous experiments have demonstrated the neuroprotective effects of intraperitoneal hydrogen saline in a neonatal hypoxia-ischemia rat model [11]
In the present study, we evaluated the neuroprotective effects of hydrogen saline against cerebral ischemia-reperfusion injury
Summary
Stroke is the second most frequent cause of death worldwide and the most frequent cause of permanent disability [1,2]. Hydrogen inhalation is not convenient and may be dangerous because it is inflammable and explosive if the concentration of hydrogen in the air is greater than 4%. After saturated in the physiological saline, molecule hydrogen in the saline is more easy to apply and safer than hydrogen inhalation. Considering the safety and the convenience, hydrogen saline has been prepared in our department and our previous experiments have demonstrated the neuroprotective effects of intraperitoneal hydrogen saline in a neonatal hypoxia-ischemia rat model [11]. Significantly improved postischemic functional recovery of rat hearts has proved after hydrogen saline treatment [12]. The present study aimed to investigate the neuroprotective effect of hydrogen saline in the rat MCAO model
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