Abstract
Background: Insulin may affect the regeneration and reconstruction of damaged skin, without affecting the blood glucose level in both diabetic people and those without diagnosed diabetes. This hormone is recommended in the prevention of diabetic foot ulcers. Objective: The aim of our research was to develop a form of insulin that could be applied to the skin. We chose hydrogels commonly used for pharmaceutical compounding, which can provide a form of therapy available to every patient. Their advantages include simple production technology, low financial costs associated with their preparation, high efficiency, high biocompatibility, a low sensitizing potential and an aesthetic and modern form of application. Methods: We prepared different gel formulations. The quality of the preparations was assessed based on measurements of physical and rheological parameters as well as insulin release in vitro. The pharmaceutical availability test was performed in a paddle apparatus (Erweka DT600, Husenstamm, Germany) in Enhancer CellTM chambers using a semi-permeable Spectra/Por 2 dialysis membrane with a pore size of 12-14 kDa. The rheological measurements were carried out using the Lamy RM 200 Touch rotational rheometer equipped with the MK-CP 2445 measuring system. The mean particle size was determined by dynamic light scattering (Litesizer 500, Anton Paar, Austria). Results: The obtained results indicate that hydrogels are promising carriers in the treatment of diabetic foot ulcers. Conclusion: The effective treatment can be achieved with a methyl cellulose-based insulin preparation.
Published Version
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