Abstract
This study used bacteria from hydrocarbon-contaminated soil that had been treated. Organic amendments included cassava peels, plantain peels, eggshells, and poultry droppings, while inorganic amendments included NPK and Urea. In this study, three (3) bacteria were characterized and dealt with: Bacillus amyloliquefaciens, Bacillus proteolyticus, and Providencia rettgeri. To determine their biodegradative capabilities, these isolates were tested on a variety of hydrocarbons. In addition, the antibacterial activity of the bacteria was tested in vitro against extracts of Moringa oleifera, Vernonia amygdalina (Bitter leaf), Ocimum gratissimum (Scent lLeaf), and Telifairia occidentalis (Ugwu) at different concentrations (20%, 13%, 10%, 7%, and 3%). Using the maceration procedure, the plant materials were extracted with ethanol and methanol. The bacteria were resistant to the various concentrations of plant extracts, according to the results. The methanolic and ethanolic extracts of Vernonia amygdalina (bitter leaf) had the maximum inhibition zone, measuring 6.00 ± 0.00 mm. Furthermore, all isolates were resistant to crude oil, engine oil, heptane, and benzene in antimicrobial tests on hydrocarbons, with Bacillus amyloliquefaciens having the highest level of susceptibility (18 mm) to pyridine. However, the bacteria all demonstrated broad spectrum susceptibility to the commercial antibiotics used as a control, with the exception of Providencia rettgeri, which proved resistant (5 mm) to Ampiclox (APX), Zinnacef (Z) and Amoxacillin (AM).
Highlights
Petroleum and its by-products pollute the discovery and use, fueled by the rapid rate of environment as a result of inappropriate waste industrialization and has resulted in increased management, poor effluent and wastewater pollution, which has invariably wreaked havoc on disposal from companies and dwellings, as well as the ecology (Alam et al, 2018)
Prior to contamination with spent engine oil, physiochemical analysis of the experimental soil and organic amendments revealed that the soil had a pH of 6.5 and a sandy loam texture when assessed on the textural triangle
The findings of this study revealed that the extraction solvents were unable to liberate some of the screened phytochemicals as steroids were absent in both the scent and bitter leaf extracts, phlobatannins were absent in all leaf extracts except Moringa and alkaloids were absent in both the bitter and scent leaf extracts
Summary
Petroleum and its by-products pollute the discovery and use, fueled by the rapid rate of environment as a result of inappropriate waste industrialization and has resulted in increased management, poor effluent and wastewater pollution, which has invariably wreaked havoc on disposal from companies and dwellings, as well as the ecology (Alam et al, 2018). Contaminants other man-made activities (Aluko et al, 2018). Oil build up in the soil, are absorbed by plant roots, Corresponding Author: Adetitun, D.
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