Abstract
Nicotiana gossei, a disease- and insect-resistance species, was hybridized with the cigar-wrapper variety of N. tabacum, ‘Florida 17’. The sterile interspecific hybrid was converted to a fertile amphiploid by treatment with colchicine. Of two methods of colchicine treatment one was more effective than the other. Certain differences in morphological details of shoot and leaf development were observed after colchicine treatment. Descriptions were also given of the relative fertility of the progenies resulting from reciprocal crosses of the recurrent parent Florida 17 by the amphiploid and by the sesquidiploid.
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