Abstract
The aim of this study is to assess if an adhesive biopolymer, sodium hyaluronate (NaHA), has synergistic effects with s-PRGF (a serum derived from plasma rich in growth factors and a blood derivative that has already shown efficacy in corneal epithelial wound healing), to reduce time of healing or posology. In vitro proliferation and migration studies, both in human corneal epithelial (HCE) cells and in rabbit primary corneal epithelial (RPCE) cultures, were carried out. In addition, we performed studies of corneal wound healing in vivo in rabbits treated with s-PRGF, NaHA, or the combination of both. We performed immunohistochemistry techniques (CK3, CK15, Ki67, ß4 integrin, ZO-1, α-SMA) in rabbit corneas 7 and 30 days after a surgically induced epithelial defect. In vitro results show that the combination of NaHA and s-PRGF offers the worst proliferation rates in both HCE and RPCE cells. Addition of NaHA to s-PRGF diminishes the re-epithelializing capability of s-PRGF. In vivo, all treatments, given twice a day, showed equivalent efficacy in corneal epithelial healing. We conclude that the combined use of s-PRGF and HaNA as an adhesive biopolymer does not improve the efficacy of s-PRGF alone in the wound healing of corneal epithelial defects.
Highlights
Integrity of the corneal epithelium is a critical requirement for correct vision function [1]
S-PRGF has already been used successfully as a treatment for eye disorders [4,16,17] and its effectiveness has been proved in wound healing [4,18]. s-PRGF has been proved to stimulate proliferation and migration of epithelial cells [18]
We studied cell proliferation at 0, 24, 48, and 72 h in rabbit epithelial cells (RPCE) and human corneal epithelial (HCE) cultures under the following treatments: 45% s-PRGF; 45% s-PRGF + 0.1% sodium hyaluronate (NaHA); 0.1% NaHA; 10% fetal bovine serum (FBS) as a positive/reference control; and 1% BSA as a negative control
Summary
Integrity of the corneal epithelium is a critical requirement for correct vision function [1]. As wound healing demands a balanced combination of different mediators, blood derivatives have been used to treat corneal epithelial defects, including autologous serum [14] and platelet rich plasma [15]. S-PRGF (a serum derived from plasma rich in growth factors) has already been used successfully as a treatment for eye disorders [4,16,17] and its effectiveness has been proved in wound healing [4,18]. It has a moderate platelet concentration and its leukocyte content has been removed [19,20]
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