Hyaluronic Acid (800 kDa) Supplementation of University of Wisconsin Solution Improves Viability of Osteochondral Grafts and Reduces Matrix Metalloproteinase Expression during Cold Preservation.

Takuya Yamada,Hisako Fujimaki,Kenji Onuma,Jun Aikawa,Masashi Takaso,Gen Inoue,Hiroyuki Sekiguchi,Shotaro Takano,Kentaro Uchida,Masayuki Miyagi

doi:10.1155/2015/631369

Takuya Yamada, Hisako Fujimaki + Show 8 more

Open Access

https://doi.org/10.1155/2015/631369

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Journal: TheScientificWorldJournal	Publication Date: Jan 1, 2015
Citations: 4	License type: CC BY 3.0

Affiliation: Kitasato University

Abstract

Osteochondral allografting is a promising option for the treatment of large cartilage defects. However, because the cell viability of osteochondral tissues (OCTs) gradually reduces during storage at 4°C, methods for maintaining the cell viability of fresh OCTs are needed to improve transplantation outcomes. Here, we evaluated whether the supplementation of preservation solution with one of three different molecular weight forms of hyaluronic acid (HA) improved the viability of rat OCTs during long-term cold storage. The supplementation of University of Wisconsin (UW) solution with 800 kDa significantly improved the cell viability of OCT after 14 days at 4°C compared to nonsupplemented UW solution. In contrast, UW solution supplemented with either 1900 or 6000 kDa HA did not markedly improve the cell viability of the OCT. Real-time PCR analysis revealed that the levels of matrix metalloproteinases 2, 3, and 9 were significantly decreased in OCT stored in UW solution supplemented with 800 kDa HA. Although further studies in human OCT are warranted, these findings demonstrate that the use of 800 kDa HA in place of serum may be a suitable approach for the long-term preservation of osteochondral allografts designated for the repair of large cartilage defects in the clinical setting.

Highlights

Osteochondral allografting is promising option for the restorative treatment of large cartilage defects caused by osteonecrosis, osteochondritis dissecans, and traumatic injury due to the low rates of donor-site morbidity
After 14 days of storage in University of Wisconsin (UW) solution supplemented with HA800 and HA6000 at 4∘C, the osteochondral tissues (OCTs) samples had significantly more normal chondrocytes and fewer degenerated chondrocytes compared with those stored in UW solution without HA800 or HA6000 (P < 0.05; Table 2)
The mean proportions of normal chondrocytes in OCTs preserved in UW solution alone and UW solution supplemented with HA1900 were 3.3% and 5.9%, respectively, whereas those in UW solution supplemented with HA800 and HA6000 were 22.4% and 15.8%, respectively

Summary

Introduction

Osteochondral allografting is promising option for the restorative treatment of large cartilage defects caused by osteonecrosis, osteochondritis dissecans, and traumatic injury due to the low rates of donor-site morbidity. Fresh osteochondral allografts typically have high functional outcomes after transplantation [1], the clinical use of this tissue is associated with the possibility of disease transmission. For this reason, OCTs are typically preserved at 4∘C during the required 14-day disease-screening period. The cell viability of OCTs gradually decreases during cold preservation, leading to marked reductions in the efficacy and clinical outcomes of cartilage repair [2]. Maintaining a high cell viability of OCT during cold storage in culture medium is necessary to improve the efficacy of osteochondral allografting. Due to the potential risk of human infection associated with the use of FBS, the development of serum-free methods that allow for the long-term

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Conclusion