Abstract
BackgroundThe invention of an effective kidney preservation solution capable of prolonging harvested kidney viability is the core of kidney transplantation procedure. Researchers have been working on upgrading the preservation solution quality aiming at prolonging storage time while maintaining utmost organ viability and functionality. For many years, the University of Wisconsin (UW) solution has been considered the gold standard solution for kidney preservation. However, the lifespan of kidney preservation in the UW solution is still limited. Its impact on the epithelial Na+ channel (ENaC) activity and its mediated processes is unknown and the primary goal of this study.MethodsKidneys harvested from 8 weeks old Sprague Dawley rats were divided into 4 groups depending upon the period of preservation in UW solution. Additional analysis was performed using dogs’ kidneys. ENaC activity was measured using patch clamp technique; protein expression and mRNA transcription were tested through Western blot and RT-qPCR, respectively. A colorimetric LDH level estimation was performed at different time points during UW solution preservation.ResultsKidney preservation in Wisconsin solution caused reduction of the kidney size and weight and elevation of LDH level. ENaC activity increased in both rat and dog kidneys preserved in the UW solution as assessed by patch clamp analysis. On the contrary, ENaC channel mRNA levels remained unchanged.ConclusionsENaC activity is significantly elevated in the kidneys during preservation in UW solution, which might affect the immediate post-implantation allograft function and trajectory post-transplant.
Highlights
The invention of an effective kidney preservation solution capable of prolonging harvested kidney viability is the core of kidney transplantation procedure
Eight-week-old Sprague Dawley (SD) rats were used to test the effects of University of Wisconsin (UW) solution
Hypothermic storage in UW solution for 24, 48, and 72 h resulted in kidney shrinkage and a significant reduction in their weights by 16, 18, and 21%, respectively (Fig. 1a and b)
Summary
The invention of an effective kidney preservation solution capable of prolonging harvested kidney viability is the core of kidney transplantation procedure. Researchers have been working on upgrading the preservation solution quality aiming at prolonging storage time while maintaining utmost organ viability and functionality. The current preservation solutions used with kidneys have reduced viability, increase delayed graft function, and higher rate of rejection that becomes more pronounced with preservation time. Most surgeons are very reluctant to transplant a kidney that has been on ice for more than 24 h This has contributed to the relatively high kidney discards ranging between 5200 and 7300 per year (United Network for Organ Sharing). Improving renal preservation methods to increase organ availability has become mandatory for covering the gap between wait-listed patients and the limited number of available organs
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