HuR Promotes the Progression of Gastric Cancer through Mediating CDC5L Expression.

Jing Cui,Nanjing Cao,Yunqing Chen,Guochao Wang,Jian Wang,Feng Li,Bin Yang,Yongqiang Lv,Fuhua Wang,Jing Zhong

doi:10.1155/2022/5141927

Abstract

Methods We performed qRT-PCR, cell cycle assay, cell migration, and mouse transplantation model analysis in our experiments. It has been clarified that HuR and microRNAs (miRNAs) have important interplays in the regulation of tumor progression. Results This study found microRNA-133b (miR-133b), as a HuR-sponged miRNA in GC cells. Downregulation of HuR can promote the expression of miR-133b and further affect the downstream cyclin CDC5L. The expressions of miR-133b were slightly lower in GC tissues than adjacent normal tissues. Conclusion Our studies suggest that HuR and miR-133b are involved in the development and pathological process of GC cells.

Highlights

Gastric cancer (GC) is one of the most common gastrointestinal tumors
We found that the expression of many miRNAs was upregulated or downregulated, among which miR-133b was most significantly downregulated in GC tissues
To determine the expression of human antigen receptor (HuR) (ELAVL1, embryonic lethal abnormal vision (ELAV) like RNA binding protein 1) in GC, 80 pairs of clinic GC tissues and matched adjacent normal tissues were analyzed with quantitative polymerase chain reaction (PCR)

Summary

Introduction

Gastric cancer (GC) is one of the most common gastrointestinal tumors. In China, the diagnosis and treatment rates of early GC are less than 10%. Clinical statistics show that the incidence and death rate of GC is high, but the early diagnosis rate and 5-year survival rate are low. Studies have shown that lymphatic metastasis of GC is an important factor affecting the prognosis of early GC [2]. It is of great clinical significance to explore the related factors of GC metastasis, early prediction of the risk of lymph node metastasis to improve the survival rate of patients. Gastric cancer (GC) is one of the common gastrointestinal tumors and the third in the mortality rate among tumors. It has been clarified that HuR and microRNAs (miRNAs) have important interplays in the regulation of tumor progression. This study found microRNA-133b (miR-133b), as a HuR-sponged miRNA in GC cells. Our studies suggest that HuR and miR-133b are involved in the development and pathological process of GC cells

Objectives

Results

Conclusion