Human-associated scarcity of hosts for tsetse flies (Diptera: Glossinidae) is related to an increase in prevalence of trypanosome infection in flies in north-eastern Zambia.

Abstract

Occurrence of nutritional stress (due to depletion of fat reserves) in tsetse flies, associated with inadequate levels of access to blood meals, enhances susceptibility of the flies to trypanosome infection. Thus, in a tsetse-infested area, a spatial gradient of reducing tsetse habitat quality is potentially a gradient of increasing prospects for occurrence of stress in tsetse flies. This study investigated prevalence of trypanosome infection in Glossina morsitans morsitans and G. pallidipes along a transect line hypothesised to represent such a gradient, in relation to the edge of the tsetse belt and distribution of human settlements. This was undertaken in three sites located in Lundazi, Mpika and Rufunsa districts, respectively, in north-eastern Zambia. Human settlement was concentrated at the edge of the tsetse belt in the Mpika and Rufunsa sites and evenly distributed along transect line in the Lundazi site. Tsetse fly samples were collected using black-screen fly rounds and Epsilon traps. Detection of trypanosome infection was by dissection and microscopy in Lundazi and Mpika sites and loop-mediated isothermal amplification (LAMP) test in Rufunsa site. Multiple logistic regression models were applied to determine whether the following factors, 'change in distance from edge of tsetse belt', 'tsetse sampling method' and 'sex of tsetse fly', had effect on 'prevalence of trypanosome infection' in the tsetse flies. Only 'increase in distance from the edge of tsetse belt' for G. m. morsitans was significantly associated with 'prevalence of trypanosome infection' in the flies, in the Mpika and Rufunsa sites. Distance was associated with reduced likelihood of infection with 'one or more subgenera of trypanosomes' and with 'Nannomonas trypanosomes', in the case of 'all sites collectively', 'Lundazi and Mpika sites collectively', Mpika site alone, and Rufunsa site alone. Per site, increase in distance entailed reduced prospects for Trypanozoon infection but only in the Mpika and Rufunsa sites. We conclude that in the Mpika and Rufunsa sites, increase in distance from human settlements entailed reduced likelihood of trypanosome infection, likely due to reducing tsetse habitat degradation, increasing availability of hosts, and hence increasing levels of nutrition and fat reserves, thus enhancing tsetse immunity against trypanosome infection.