Human Umbilical Mesenchymal Stem Cells-Seeded Bladder Acellular Matrix Grafts for Reconstruction of Bladder Defects in a Canine Model

Haichao Yuan,Wei Zhi,Qiang Wei,Ping Han,Liangren Liu,Ju Xiong,Yue Zhuang,Irina Kerkis

doi:10.1371/journal.pone.0080959

Haichao Yuan, Wei Zhi + Show 6 more

Open Access

https://doi.org/10.1371/journal.pone.0080959

Copy DOI

Abstract

BackgroundThe goal of this study was to explore the feasibility of utilizing human umbilical mesenchymal stem cells (HUMSCs)-seeded Bladder acellular matrix graft (BAMG) for bladder reconstruction in a canine model.Methodology/Principal FindingsHUMSCs were isolated from newborn umbilical cords and identified by flow cytometry. Partial cystectomy was performed in the experimental and control group. Bladder defects were repaired with HUMSCs-BAMG in the experimental group and repaired with unseeded-BAMG in control group. The implanted grafts were harvested after surgery. H&E and immunohistochemistry staining were performed to evaluate the regeneration of the bladder defect. Primary cultured HUMSCs displayed typical fibroblast morphology with spindle-shaped. Flow cytometry indicated that these cells were positive for CD105 (97.3%) and CD44 (99%), but negative for CD34 (2.8%), CD31 (2.1%), and CD45 (1.7%). Immunohistochemistry staining showed that a multilayered urothelium and well-developed smooth muscle were observed at 12 weeks in experiment group. In contrast, multilayered urothelial tissues were also observed at 12 weeks in group B, but well-developed smooth muscle bundles were observed.Conclusions/SignificanceOur preliminary results demonstrate that UMSC-seeded BAMGs are superior to unseeded BAMGs to promote the regeneration of bladder defects. Our findings indicated that HUMSCs may be a potential cell source for bladder tissue engineering.

Highlights

The repair of bladder defects caused by trauma or tumors is often problematic and poses a serious challenge for urological surgeons [1]
Conclusions/Significance: Our preliminary results demonstrate that Umbilical mesenchymal stem cells (UMSCs)-seeded Bladder acellular matrix graft (BAMG) are superior to unseeded BAMGs to promote the regeneration of bladder defects
Our findings indicated that human umbilical mesenchymal stem cells (HUMSCs) may be a potential cell source for bladder tissue engineering

Summary

Introduction

The repair of bladder defects caused by trauma or tumors is often problematic and poses a serious challenge for urological surgeons [1]. The development of tissue engineering techniques will bring new opportunities for bladder reconstruction [2]. These techniques involve seeding biomaterial scaffolds with appropriate cells in the laboratory and implanting them in vivo to repair or regenerate damaged tissue [3]. Certain studies have reported that transplantation of biomaterial seeded with autologous urothelial and smooth muscle cells could allow for the regeneration of a functional bladder in several animal models [4,5,6]. The goal of this study was to explore the feasibility of utilizing human umbilical mesenchymal stem cells (HUMSCs)-seeded Bladder acellular matrix graft (BAMG) for bladder reconstruction in a canine model

Objectives

Methods

Results

Conclusion