Human Skin Penetration and Distribution via Different Vesicular Systems

Abstract

Aim s: This Study was performed to evaluate the quantitative distribution of mannitol (model hydrophilic molecule) and corticosterone (model lipophilic molecule) in human skin following the application of different vesicular systems (liposome, ethosome and invasome). Methodology: Vesicular systems were prepared by thin film hydration method and particle size, particles distribution, zeta potential and in vitro human skin penetration and skin deposition were evaluated. Results and Discussion: Results showed that all of the investigated vesicular systems had almost nanometric size ranging from 67.46±0.25nm and 108.3±0.95nm with low polydispersity (PDI <0.2). All vesicular systems exhibited negative zeta potential. In vitro skin deposition of corticosterone showed that invasome had significant lower accumulation than ethosome and liposome in SC (by ~ 5 and 4 folds respectively) while showed only lower accumulation than liposome in epidermis. Ethosome showed insignificant higher accumulation than CL-CS in both SC and epidermis.