Abstract
Summary A protein with a potency of 22 IU/mg in the pigeon crop sac assay for prolactin but only 0.4 USP U/mg in the tibial line assay for growth hormone has been isolated from pooled human pituitary glands. When tested by radioimmunoassay for human growth hormone, 12 ng of the material was equivalent to 1 ng of a reference growth hormone. The protein was less electronegative than growth hormone at pH 9.5 and had a molecular weight of about 22,000.
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