Human Papillomavirus Integration and its Role in Cervical Malignant Progression

Abstract

High risk Human Papilloma Virus (HR-HPV) DNA integration into the human genome is one of the key stages in the progression of cervical neoplasia. This mini-review highlights the importance of HPV integration as a potential biomarker for cervical screening and briefly describes the main methods used to detect HPV integration: Amplification of Papillomavirus Oncogene Transcripts (APOT), Restriction Site PCR (RS-PCR), Southern blot and Detection of Integrated Papillomavirus Sequences (DIPS). The potential mechanisms of HPV integration are discussed with a focus on DNA instability; site of integration; and transcriptional regulation of integrants. This article provides an overview of the role HPV integration plays in malignant progression.