Human papillomavirus infection and its relationship with common polymorphism of HLA gene by PCR method

Abstract

Studies have shown that human papillomavirus (HPV) causes cervical cancer, but investigations on the relationship between HPV and HLA gene polymorphism are limited.Objective: The purpose of this study is to investigate the prevalence of HPV infection and its relationship with common HLA gene polymorphisms in cervical cancer patients using the PCR method. This study used 52 fixed samples from the laboratory of Khatam al-Anbia Hospital in Tehran. DNA was isolated using proteinase K and a DNPTM commercial extraction kit. The PCR technique was used to identify L1, HPV-16, HPV-18, HPV-31, HPV-11, HPV-33, and HPV-35 genes. The ARMS-PCR method was used to determine the genotypes of common HLA polymorphisms. The data were analyzed using one-way analysis of variance. The ARMS-PCR results showed that 10 polymorphisms were observed in the HLA gene in the samples, with 7 related to DRB1, 2 related to DQB1, and 1 related to DQA1. However, the desired polymorphisms were not observed in 42 of the studied samples. Polymorphism of DRB1 genes in 7 samples showed that 6 of these 7 samples were infected with HPV16 and one with HPV18. The study results suggest that HPV16 and HPV18 are the most common HPV types isolated from cervical cancer patients. HLA gene polymorphism may predispose people to these infections. Understanding these relationships in different populations and races is essential for understanding the role of HLA gene polymorphism in cervical cancer and its relationship with HPV infection.