Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota

Antonio Rubio-Del-Campo,María J Yebra,María Carmen Collado,Cristina Alcántara,Jesús Rodríguez-Díaz

doi:10.1038/s41598-020-68718-4

Antonio Rubio-Del-Campo, María J Yebra + Show 3 more

Open Access

PDF Available

https://doi.org/10.1038/s41598-020-68718-4

Copy DOI

Export

Save

Cite

Abstract
Highlights/Summary
Full-Text PDF
Similar Papers

Abstract

Listen

Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition. The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides. We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota. LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum. The Lactobacillus genus levels were increased by 3FN fermentation and B. breve by GNB and 3FN. There was a significant reduction of Blautia coccoides group with LNB and 3FN. In addition, 6FN significantly reduced the levels of Enterobacteriaceae family members. Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures. Additionally, after fermentation of the oligosaccharides by the fecal microbiota, several Bifidobacterium strains were isolated and identified. The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.

Highlights

Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition
The results presented here (p = 0.0964, Dunnett’s test) (Fig. 1a) and previous studies, using fecal bacteria from formula-fed infants, showed that the total bifidobacterial community incremented with LNB12
Studies using in vitro analysis and animal models suggested that human milk oligosaccharides (HMOs) are crucial for modulating the infant gut microbiota and exerting important health benefits, including prevention of pathogen attachment and immunomodulation[2,35,36,37]

Summary

Introduction

Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition. Total HMOs purified from human milk increased the number of Bifidobacterium spp. while the number of Escherichia spp. and Clostridium perfringens diminished during in vitro fermentation of infant microbiota[1]. In these cultures, most of the 2′FL, 3-fucosyllactose (3FL) and lactodifucotetraose (LDFT) from the HMOs supplement were consumed, suggesting that some specific oligosaccharides may be responsible of the prebiotic effect attributed to HMOs. A few studies using in vitro colon m odels[14] and in vivo animal models underline the ability of individual HMOs to modify the composition of the gastrointestinal microbiota[15,16]. Our final aim is to compare the potential prebiotic effect of those four disaccharides

Methods

Results

Conclusion