Abstract
BackgroundRadiation dermatitis is a refractory skin injury caused by radiotherapy. Human fetal skin-derived stem cell (hFSSC) is a preferable source for cell therapy and skin tissue regeneration. In the present study, we investigated the repair effect of using hFSSC secretome on a radiation skin injury model in rats.MethodsWe prepared the hFSSC secretome and studied its effects on the proliferation and tube formation of human umbilical vein endothelial cell (HUVEC) in vitro. Furthermore, we used a Sr-90 radiation-induced skin injury model of rats and evaluated the effects of hFSSC secretome on radiation skin injury in vivo.ResultsThe results showed that hFSSC secretome significantly promoted the proliferation and tube formation of HUVEC in vitro; in addition, hFSSC secretome-treated rats exhibited higher healing quality and faster healing rate than the other two control groups; the expression level of collagen type III α 1 (Col3A1), transforming growth factor β3 (TGF-β3), angiotensin 1 (Ang-1), angiotensin 2 (Ang-2), vascular endothelial growth factor (VEGF), and placental growth factor (PLGF) was significantly increased, while collagen type I α 2 (Col1A2) and transforming growth factor β1 (TGF-β1) were decreased in hFSSC secretome group.ConclusionsIn conclusion, our results provided the first evidence on the effects of hFSSC secretome towards radiation-induced skin injury. We found that hFSSC secretome significantly enhanced radiation dermatitis angiogenesis, and the therapeutic effects could match with the characteristics of fetal skin. It may act as a kind of novel cell-free therapeutic approach for radiation-induced cutaneous wound healing.
Highlights
Radiation dermatitis is a refractory skin injury caused by radiotherapy
The results showed that Human fetal skin-derived stem cell (hFSSC) were strongly positive for surface markers, such as CD73, CD90, CD105, Oct4, and Sox2 (Fig. 1)
HFSSC secretome promoted human umbilical vein endothelial cell (HUVEC) proliferation and tube formation in vitro As shown in Fig. 2a, b, we found that compared to the other two control groups, hFSSC secretome had the highest percentage of Ki-67-positive cells in HUVEC (SFM 15.2%, human umbilical cord mesenchymal stem cell (hUCMSC) secretome 72.3%, and hFSSC secretome 88.4%)
Summary
Radiation dermatitis is a refractory skin injury caused by radiotherapy. Human fetal skin-derived stem cell (hFSSC) is a preferable source for cell therapy and skin tissue regeneration. We investigated the repair effect of using hFSSC secretome on a radiation skin injury model in rats. Variable degrees of damage often occur in skin tissues during radiotherapy [2]. Recent studies have shown stem cells to be a promising strategy to treat refractory skin damage [5]. The human fetal skin-derived stem cell (hFSSC), which is the early stage of adult stem cells derived from 8 to 12 weeks fetus of spontaneous abortion, was used [6]. Fetal skin tissue which is donated with consent for research is processed in vitro, confirming cell function without genetic abnormality and contamination [7]. Fetal tissue contains a large number of stem cells and progenitor cells for development, making it
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