Abstract
Conformers of pyruvate kinase (ATP: pyruvate-phosphotransferase, EC 2.7.1.40) could be consistently obtained with pI values of 5.28 and 6.70. These were converted to a form with a pI value of about 5.86 by (D)-1,6-fructose diphosphate. Still another conformer with a pI value of about 7.2 was obtained in some preparations electrofocused in the absence of 2-mercaptoethanol. Re-electrofocusing of the higher pI material resulted in conversion of some to the form with a pI value of 5.3. The forms with pI values of 5.3 and 6.7 were subjected to sedimentation and kinetic studies. The two conformers differed kinetically from each other and from the sample which had not yet been subjected to electrofocusing.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
