Human Bocavirus in Hospitalized Children, South Africa

Heidi Smuts,Di Hardie

doi:10.3201/eid1209.051616

Abstract

Human Bocavirus in Hospitalized Children, South Africa

Highlights

To the Editor: In recent years, several novel respiratory viruses have been identified
341 nasopharyngeal and bronchoalveolar lavage samples were taken from children hospitalized with respiratory tract infections in 2004 in the Red Cross War Memorial Children’s Hospital, Cape Town, South Africa
Samples were originally screened by using an indirect immunofluorescence assay (Light Diagnostics, Chemicon International, Temecula, CA, USA) for common respiratory viruses, including respiratory syncytial virus; influenza virus A and B; parainfluenza viruses 1, 2, and 3; adenovirus; and cytomegalovirus

Summary

Introduction

To the Editor: In recent years, several novel respiratory viruses have been identified. 341 nasopharyngeal and bronchoalveolar lavage samples were taken from children (age 2 days–12 years) hospitalized with respiratory tract infections in 2004 in the Red Cross War Memorial Children’s Hospital, Cape Town, South Africa. Samples were originally screened by using an indirect immunofluorescence assay (Light Diagnostics, Chemicon International, Temecula, CA, USA) for common respiratory viruses, including respiratory syncytial virus; influenza virus A and B; parainfluenza viruses 1, 2, and 3; adenovirus; and cytomegalovirus. PCR amplification of a region of the NP-1 gene and the 3′ portion of the VP1/2 capsid gene of HBoV was performed.

Results

Conclusion