Abstract
Human Bocavirus DNA in Paranasal Sinus Mucosa
Highlights
To the Editor: Human bocavirus (HBoV) is a newly described parvovirus for which pathogenic potential has not clearly been elucidated [1]
25 mg of each tissue specimen was used for nucleic acid extraction by using an RNeasy Mini Kit, as described [5] (QIAGEN, Hamburg, Germany)
To provide evidence that the QIAGEN RNeasy kit is suitable for DNA extraction, we spiked HBoV negative samples with different amounts of HBoV DNA before extraction of nucleic acids was done with either the QIAGEN RNeasy Kit or DNA Blood Kit (QIAGEN)
Summary
To the Editor: Human bocavirus (HBoV) is a newly described parvovirus for which pathogenic potential has not clearly been elucidated [1]. We analyzed samples of paranasal mucosal tissue and nasal polyps from patients with chronic sinusitis for respiratory viruses and atypical bacteria. A total of 102 tissue samples were obtained from 88 patients (median age 48.5 years, range 13.3– 88.1 years) from July 2009 through September 2010 after elective surgery. To detect asymptomatic shedding in the upper respiratory tract and viremia, we collected nasal swabs and EDTA-blood samples concurrently.
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