Http://www.plantarchives.org/SPECIAL%20ISSUE%2021-1/429%20(2636-2640).pdf

Abstract

In the present study, Fifty random samples of each small-scale (plain yoghurt and kariesh cheese) were collected randomly from different dairy shops and markets in Zagazig city for isolation and identification of Escherichia coli which is considered a good indicator of faecal contamination and a major cause of food poisoning. Identification was done microscopically and biochemically by different biochemical tests (IMVIC).The incidence of E. coli in yoghurt and kariesh cheese samples were 36% and 50%, respectively. Also, the serological identification of E. coli isolates revealed that O26 is the most predominant serogroup by percentagesof27.77% and 28% in both yoghurt and kariesh cheese samples, respectively. Because of its rising resistance to antibiotics, E.coli represents a real threat to human health. Antimicrobial susceptibility testing (AST) was done by disc diffusion method against 10 antimicrobials and the results revealed that E. coli isolates were highly resistant to amoxicillin-clavulanate, ampicillin, Cefotaxime and Ceftazidime, and were highly sensitive to chloramphenicol, Ciprofloxacin and tetracycline. In addition, 83.72%of E. coli isolates showed multi drug resistance (MDR).Bacterial adhesion to food surfaces and the formation of biofilmisa source of food contamination that has an impact on food safety and industry. Micro titer plate assay was used for testing biofilm formation and revealed that 69.77% of E. coli isolates were non-biofilm producers, 9.30% were weak biofilm producers, 20.93%were moderate biofilm producers and none of isolates was strong biofilm producers. Virulence genes of E. coli isolates were identified and characterized by a multiplex PCR assay. The results showed that among the target genes, stx1was most frequently detected by a percentage of81.82%