Abstract
In the present study, we have examined if there is any difference in biofilm production among different genotypes of Pseudomonas aeruginosa. The study investigated 526 non-duplicate P. aeruginosa isolated from clinical specimens and from a hospital environment. Isolates were grouped into thirty-five genotypes based on an identical ERIC2-band pattern. Biofilm formation was quantified by the microtiter plate test and all strains were classified into the following categories: no biofilm producers (0), weak (+), moderate (+), or strong (+++) biofilm producers. Only 2.45% of examined strains were not biofilm producers. Among biofilm producers, 39.26% were strong biofilm producers, 34.36% were moderate biofilm producers, while 23.93% were weak biofilm producers. Although the majority of strong biofilm producers were in genotype groups 2 and 3, the degree of in vitro biofilm formation in our study was not significantly affected by the genotype of Pseudomonas aeruginosa. In this study, we demonstrated that the degree of in vitro biofilm formation is not significantly affected by the genotype of Pseudomonas aeruginosa.
Highlights
Pseudomonas aeruginosa, which is predominantly an environmental bacterium, is an opportunistic pathogen that accounts for 10% of nosocomial infections
We examined and compared the biofilm formation of 163 Pseudomonas aeruginosa isolates in 96well polystyrene microtiter plates
Over the last couple of decades, much evidence has supported the hypothesis that biofilms play a large role in the pathogenesis of chronic infections (Moreau-Marquis et al, 2010)
Summary
Pseudomonas aeruginosa, which is predominantly an environmental bacterium, is an opportunistic pathogen that accounts for 10% of nosocomial infections. Infection generally depends on the host having a compromised immune system (Rutherford and Bassler, 2012). Pseudomonas aeruginosa is wonderfully adept at forming highly organized surfaceassociated communities encased within an exopolysaccharide and protein matrix, known as biofilms. Bacterial biofilms are surface-associated, multicellular, morphologically complex microbial communities (Zhao et al, 2013). Bacterial cells and a wide range of self-generated extracellular polymeric substances (EPS). Biofilm formation is shown to be coordinated by EPS production, cell migration, subpopulation differentiation and interactions (Yang et al, 2011)
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