HPV detection using primers MY09/MY11 and GP5+/GP6+ in patients with cytologic and/or colposcopic changes

Emanuella Meneses Venceslau,Anna Carolina Mota Lopes,Mauro Muniz Bezerra,Fabiana Botelho De Miranda Onofre,Érick Vieira Souza,Alexandre Sherlley Casimiro Onofre,Claudia Moura De Melo,Verônica De Lourdes Sierpe Jeraldo

doi:10.5935/1676-2444.20140028

Emanuella Meneses Venceslau, Anna Carolina Mota Lopes + Show 6 more

Open Access

https://doi.org/10.5935/1676-2444.20140028

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Abstract

Introduction: Cervical cancer is one of the most common diseases among women, and cause considerable morbidity and mortality. Considering that cervical cancer is an important neoplasia in northeastern Brazil, and the prevalence of high-risk human papillomavirus (HPV) is directly associated with it, this work had aimed to correlate the cytological and/or colposcopic findings with HPV infection status, and verify the performance of MY09/MY11 and GP5+/6+ primers for HPV detection. Material and method: Patients in this study were from Penedo-AL, a city with high level of poverty (poverty rate of 60.62%). Out of 70 patients with cytological and/or colposcopic changes, 32 agreed to participate in the study. Results: Regarding cytology, 21 (30%) patients presented atypical squamous cells of undetermined significance (ASC-US); 20 (29%), atypical glandular cells of undetermined significance (AGUS); 12 (17%), low-grade intraepithelial lesion (LSIL); five (7%), high-grade intraepithelial lesion (HSIL); and 12 (17%), positive colposcopy. From these, 27 (84%) presented the band gene encoding for human s-globin. From the 27 patients, eight (30%) were positive for HPV. The results showed that the deoxyribonucleic acid (DNA) of HPV was detected in 15% and 30% by using MY-PCR and GP +-PCR, respectively. Conclusion: This study suggests that more than one type of oligonucleotide primer should be used in clinical samples to increase sensitivity for the detection of HPV.

Highlights

Cervical cancer is one of the most common diseases among women, and cause considerable morbidity and mortality
Results showed that deoxyribonucleic acid (DNA) of human papillomavirus (HPV) was detected in 15% and 30%, using MY-polymerase chain reaction (PCR) and GP+-PCR, respectively
21 patients (30%) showed atypical squamous cells of undetermined significance (ASC-US); 20 (29%), atypical glandular cells of undetermined significance (AGUS); 12 (17%), LSIL; and five (7%), HSIL

Summary

Introduction

Cervical cancer is one of the most common diseases among women, and cause considerable morbidity and mortality. Considering that cervical cancer is an important neoplasia in northeastern Brazil, and the prevalence of high-risk human papillomavirus (HPV) is directly associated with it, this work had aimed to correlate the cytological and/or colposcopic findings with HPV infection status, and verify the performance of MY09/MY11 and GP5+/6+ primers for HPV detection. According to Globocan (2008), 188 thousand new cases occur annually in Asia, 134 thousand in India, 75 thousand in Africa, and 24 thousand in Brazil. In developed countries, such as the United States of America, the incidence was 11 thousand, and in the European Union was 31 thousand[10]. The differences in incidence and mortality between developed and developing countries may be due to regional differences, prevalence and distribution of major risk factors, practices of detection and/or availability and use of treatment services[1]

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