HPTLC-Densitometric Analysis of Eperisone Hydrochloride and Paracetamol in Their Combined Tablet Dosage Form

Nirav Uchadadiya,Falgun Mehta,Pinak Sanchaniya

doi:10.1155/2013/464796

Abstract

A simple, precise, accurate, and reliable HPTLC method has been developed and validated for the analysis of EPE-Eperisone hydrochloride and PCM-Paracetamol in their combined dosage form. Identification and analysis were performed on 100 mm × 100 mm layer thickness 0.2 mm, precoated silica gel G60-F254 aluminum sheet, prewashed with methanol, and dried in an oven at 50°C for 5 min. Toluene : methanol : ethyl acetate : glacial acetic acid (4 : 3.5 : 2.5 : 0.05) (v/v/v/v) was used as mobile phase. Calibration plots were established showing the dependence of response (peak area) on the amount chromatographed. The validated calibration ranges were 200–700 ng/spot and 1300–4550 ng/spot for EPE and PCM with correlation coefficient (R2) 0.994 and 0.996, respectively. Average % recovery was between 98.61–100.94% and 99.18–100.57% for EPE and PCM, respectively. The spots were scanned at 248 nm in a reflectance mode. The proposed method was validated as per ICH guidelines and successfully applied to the estimation of EPE and PCM in their combined tablet dosage form.

Highlights

EPE is chemically (2RS)-1-(4-ethylphenyl)-2-methyl-3-(1-piperidinyl)propan-1-one and hydrochloride (1 : 1) (Figure 1(a)) [1, 2]
Eperisone HCl increases the blood supply to skeletal muscles; this action is noteworthy since a muscle contracture may compress the small blood vessels and induce an ischemia leading to release of pain stimulating compounds [3]
The present paper describes a simple, accurate, and precise method for simultaneous estimation of EPE and PCM in combined tablet dosage form by HPTLC method

Summary

Introduction

EPE is chemically (2RS)-1-(4-ethylphenyl)-2-methyl-3-(1-piperidinyl)propan-1-one and hydrochloride (1 : 1) (Figure 1(a)) [1, 2]. Eperisone HCl is a centrally acting muscle relaxant; it acts at the level of spinal cord by blocking sodium channels and calcium channels. Eperisone HCl exerts its spinal reflex inhibitory action predominantly via a presynaptic inhibition of the transmitter release from the primary afferent endings via a combined action on voltage-gated sodium and calcium channels. EPE is official in Japanese pharmacopoeia [1]. Paracetamol is a weak inhibitor of PG-Prostaglandin synthesis of COX-1 and COX-2. Paracetamol is official in Japanese Pharmacopoeia [1], British Pharmacopoeia [5], United States Pharmacopoeia [6], and Indian Pharmacopoeia [7]

Methods

Results

Conclusion