Abstract
An HPLC-PDA method was developed and validated for the determination of hydrochlorothiazide in bulk and pharmaceutical formulation. The method was optimized selecting chromatographic conditions of 50 : 50 acetonitrile : water, Inertsil® column (ODS-3 250 mm × 4.6 mm 5 μm), 20 μL injection volume, flow rate of 1 mL/min at ambient temperature (30°C), and 272 nm. Another column of C18Zorbax® (Eclipse Plus, 4.6 × 250 mm, 5 μm) was tested showing no big difference in the method results. The method was validated giving good precision (RSD% < 1), acceptable linearity (R2 ≥ 0.9978), and low LOD and LOQ (0.5 and 1.7 μg/mL, resp.) on both columns. Successful application on pharmaceutical dosage tablet form gave high recovery of 99.93%. The method was compared with official BP and other reported methods. The proposed method is economic, simple, and rapid and hence can be employed for routine analysis in quality control laboratories.
Highlights
Hydrochlorothiazide chemical name is 2H-1,2,4-Benzothiadiazine-7-sulfonamide,6-chloro3,4-dihydro,1,1-dioxide (Figure 1)
Hydrochlorothiazide belongs to a class of drugs called thiazide diuretics antihypertensive [1]
A recent study involved a review of developing methods using these analytical techniques for estimation of hydrochlorothiazide in various biological samples with focus on sample pretreatment methods and separation methods [10]
Summary
Hydrochlorothiazide chemical name is 2H-1,2,4-Benzothiadiazine-7-sulfonamide,6-chloro3,4-dihydro-,1,1-dioxide (Figure 1). It is slightly soluble in water and sparingly soluble in acetonitrile. Hydrochlorothiazide binds to and inhibits the enzyme carbonic anhydrase. It is frequently used alone or in combination with other medications for the treatment of hypertension, congestive heart failure, symptomatic edema, diabetes insipidus, renal tubular acidosis, hypoparathyroidism, and edema and prevention of kidney stones and used in the treatment of osteoporosis [2]. An extensive literature survey was carried out and found that there are few analytical methods for the determination of hydrochlorothiazide alone or in its combination with other drugs in pharmaceutical preparations including spectrophotometry [3, 4], flow injection [5], TLC [6], and HPLC [7,8,9]. A recent study involved a review of developing methods using these analytical techniques for estimation of hydrochlorothiazide in various biological samples with focus on sample pretreatment methods and separation methods [10]
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