HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa

Daya L. Chothani,M. B. Patel,S. H. Mishra

doi:10.1155/2012/180103

Daya L. Chothani, M. B. Patel + Show 1 more

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https://doi.org/10.1155/2012/180103

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Abstract

The present study was aimed to identification, isolation, and quantification of marker in R. tuberosa (Acanthaceae). HPTLC fingerprinting was carried out for various extract of root, stem, and leaf of R. tuberosa. From the HPTLC fingerprint the florescent band (under 366 nm) at : 0.56 (mobile phase chloroform : toluene : ethyl acetate (6 : 3 : 1, v/v)) was found in leaf, root, and stem of R. tuberosa. So, the florescent band (under 366 nm) at : 0.56 was isolated as marker compound RT-F2 from root of R. tuberosa. The marker compound RT-F2 was quantified by using HPTLC technique. The percentage (W/W) amount of RT-F2 was found to 40.0% and 44.6% in petroleum ether and ethyl acetate extract of R. tuberosa roots, respectively. Further study is suggested to characterization and biological nature of marker compound.

Highlights

Marker compound means chemical constituents within a medicinal that can be used to verify its potency or identity
HPTLC fingerprint showed that purple colored band (Figure 3) at R f : 0.56 was found in leaf, root, and stem of R. tuberosa
The percentage (W/W) amount of RT-F2 was found to 40.0% and 44.6% in petroleum ether and ethyl acetate extract of R. tuberosa roots, respectively (Tables 1 and 2)

Summary

Introduction

Marker compound means chemical constituents within a medicinal that can be used to verify its potency or identity. The marker compounds may be described as active ingredients or chemicals that confirm the correct botanical identity of the starting material. A chromatographic fingerprint of a herbal medicine is a chromatographic pattern of the extract of some common chemical components of pharmacologically active and/or chemical characteristics. The authentication and identification of herbal medicines can be accurately conducted even if the amount and/or concentration of the chemically characteristic constituents is not exactly the same for different samples of drug. It is very important to obtain reliable chromatographic fingerprints that represent pharmacologically active and chemically characteristic component of the herbal drug [1,2,3,4,5]

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