HPTLC Analysis with the Effect ofBacopa monnieri, Evolvulus alsinoidesandTinospora cordifoliaagainst Scopolamine-Induced Amnesic Rats

Abstract

Background and Objective:Bacopa monnieri, Evolvulus alsinoides, and Tinospora cordifolia are well known herbal Ayurvedic medicinal plants with a memory enhancing property. The aim of this study was HPTLC analysis for evaluating the effect of ethanolic extract of Bacopa monnieri (BME), Evolvulus alsinoides (EAE) and Tinospora cordifolia (TCE) alone and in combination of equal proportion (CEPs) against scopolamine-induced amnesic rats.Methods:HPTLC analysis was done to evaluate the presence of phytoconstituents in these plants. Morris water maze and passive avoidance tests were also used to evaluate the effect on memory function. All pretreatment with BME, EAE, TCE, and CEPs was done at 200 mg/kg. The observation was done on the basis of the effect on mean latency time (in seconds) in the Morris water maze test and latency to reach shock free zone (SFZ), mistakes in 15 minutes on the Passive-avoidance test.Results:It was observed that after HPTLC analysis, Bacoside A (Rf value; 0.82) found in BME, flavonoid glycoside (Rf value; 0.31) found in EAE and unknown compounds (Rf value; 0.11, 0.39) observed in TCE. BME, EAE, TCE and their combinations of equal proportion [CEP-1 (BME+EAE), CEP-2 (BME+TCE), CEP-3 (EAE+TCE) and CEP-4 (BME+EAE+TCE)] produced significant effects against scopolamine-induced amnesia in rats.Conclusion:These findings suggested that the content of extracts of these compounds has an antioxidant property in the brain. Also, BME, EAE, and TCE in the CEPs provide a synergistic effect on behavioral assessment.