HPRT gene mutation, sister chromatid exchange and comet assay in peripheral lymphocytes of radiation laborers at Al-Amal Cancer Hospital in Baghdad

Maha Tareq Hussein Maha Tareq Hussein,Abdulsahib Kadhim Ali Abdulsahib Kadhim Ali,Amel Jabbar Mutta Amel Jabbar Mutta

doi:10.53771/ijbpsa.2022.3.1.0102

Maha Tareq Hussein Maha Tareq Hussein, Abdulsahib Kadhim Ali Abdulsahib Kadhim Ali + Show 1 more

Open Access

https://doi.org/10.53771/ijbpsa.2022.3.1.0102

Copy DOI

Abstract

A Study performed on 30 Iraqi radiation Labors had been exposed tо a low dose tо ionizing radiation at Al-Amal Cancer Hospital in Baghdad the use of three genetic endpoints. Covered 12 females and 18 males between the aged tо (22-57) years. In addition to 20 Healthy individuals from the population, dwelling Baghdad, covered, 7 females and 13 males, aged (19 - 55) years which are non-smokers, non- alcoholic. The cytogenetic analysis, including, HPRT gene mutation¸ comet assay and SCE have been carried out on peripheral blood lymphocytes of labors and control groups. The HPRT gene mutation assay showed a significant increase (p<0.05) in the radiation labors. There were found a significant increase (p<0.05) in comet tail length and tail moment values in thе human lymphocyte in these radiation Labors compared with thе control group. Also the SCE in human lymphocyte for radiation Laborers was significantly (p<0.05) higher than in a control group. Results obtained confirmed the cytogenetic analysis; including, HPRT gene mutation¸ comet assay and SCE are useful as biodosimetric markers for the detection of human exposure to low doses of ionizing radiation. It reflects the simultaneous exposure and actual levels of DNA damage present in radiological laborers' peripheral blood leukocytes by detecting temporary DNA damage and / or repair activity.

Highlights

The ionizing radiation is an omnipresent environment, a physical agent with very well-known damaging effects of DNA
Hypoxanthine guanine phosphoribosyl transferase (HPRT) gene mutation alkaline comet and SCEs assay were chosen as an exposure biomarker to assess continuou s exposure to ionizing radiation of 30 radiation labors occupationally exposed to ionizing radiation and 20 controls
The result of the HPRT gene mutation assay in labors of Al-Amal Hospital for Cancer at Baghdad shown in table (1)

Summary

Introduction

The ionizing radiation is an omnipresent environment, a physical agent with very well-known damaging effects of DNA. Levels of exposure to ionizing radiation in hospitals have reduced in current a long time and are properly beneath the regulatory restriction of 20 mSv / year, and in reality nicely under the detection restrict for dosimeters. Such as nuclear medicine makes use of of radiation, and interventional procedures, might expose persons to greater doses, and these are the troubles of problems of concern [1]. Several studies of Hypoxanthine guanine phosphoribosyl transferase (HPRT) gene mutations in human cultured cells and lymphocytes in vivo have provided evidence for age, exposure and genetics influence mutation frequency [7]. Acquired in children and adults who have been exposed to both known and unknown environmental mutations [10, 11]

Objectives

Methods

Results

Conclusion