HPLC-UV Analysis and Antioxidant Activities of Phenolic Compounds from Bark of Tamarix articulata vahl Shrubs Grown in Mosul Province.

Abstract

Analytical trial was carried out to investigate phenolic compounds of Tamarix articulata (vahl.) Karst shrubs were extracted from dry powdered bark with different solvents: Ethyl acetate; Industrial methylated spirits (IMS) and distilled water, and their antioxidant activity were assayed via in vitro radical scavenging activity using DPPH assay. HPLC analysis showed that there were at least 6 remarkable phenolic compounds identified of bark, which included: (Apigenin, Caffeic acid, Chlorogenic acid, kaempferol, Quercetin and Rutin). Furthermore, both of crude extracts IMS and distilled water was better than crude ethyl acetate extract for extracting the bioactive compounds particularly the number and concentration of phenolic compounds identified in bark. The crude extract of IMS achieved the highest total concentration of 0.471 (mg g−1), followed by the crude extract for hot water was 0.226 (mg g−1) and the lowest crude extract for ethyl acetate was 0.031 (mg g−1). However, all the phenolic compounds separated from the crude extracts were free radical inhibitors of DPPH but both the IMS crude extract and ethyl acetate at the same concentration of 200 (µ ml−1), and at rates of (96.7% and 96) (%), respectively, were significantly superior, on the phenolic compounds separated from the aqueous crude extract. Additionally, it’s superior on the standard sample.