Abstract
High-Performance Liquid Chromatography (HPLC) was developed for the standardization and quantification of gallic acid in the methanolic extract of Acrocarpus fraxinifolius Weight & Arn leaves. Linearity was observed in the range of 0.4–2 mg/mL with a correlation coefficient (r2) of 0.9978. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0054 mg/mL and 0.0163 mg/mL, respectively indicating the sensitivity of the applied method. Recovery values of 100.377 % indicate the best accuracy of the method. Gallic acid content was quantified as 168.75 ± 1.05 mg/g methanolic extract. This developed method was simple, accurate and precise to be used as a reference standard method for determination of gallic acid content in the plant extract.
Highlights
Standardization of herbal extracts is considered as a great important tool for quality control in production and manufacturing process of herbal drugs to define the chemical profile of the product and to assess consistent biological activity [1]
The literature showed that many galloylated compounds such as 2,3 digalloyl-α,β-glucoside, quercetin-(2''-galloyl-)3-O-β-glucopyranoside, myrecetin-(2''-galloyl-)3-O-β -glucopyranoside as well as gallic acid were isolated from the Acrocarpus fraxinifolius Weight & Arn extract [8]
The method was validated in terms of accuracy, precision, repeatability, reproducibility, limit of detection (LOD), and limit of quantification (LOQ) and illustrated in table 2
Summary
Standardization of herbal extracts is considered as a great important tool for quality control in production and manufacturing process of herbal drugs to define the chemical profile of the product and to assess consistent biological activity [1]. The validation parameters of analytical separation method are accuracy, precision, linearity, range, detection limit and quantification limit [2,3,4]. The importance of calibration linearity in the context of the quantification limit is considered, since the estimated concentrations of this limit, are deduced from the regression line. The literature showed that many galloylated compounds such as 2,3 digalloyl-α,β-glucoside, quercetin-(2''-galloyl-)3-O-β-glucopyranoside, myrecetin-(2''-galloyl-)3-O-β -glucopyranoside as well as gallic acid were isolated from the Acrocarpus fraxinifolius Weight & Arn extract [8]. S. El-Nashar, et al Arch Pharm Sci 1(1): 5-8 conducted to obtain a valid method for standardization and quantification of gallic acid in the methanolic extract of Acrocarpus fraxinifolius leaves using HPLC
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Archives of Pharmaceutical Sciences Ain Shams University
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
