HPLC-DAD Phenolic Composition, Antioxidant, Anticholinesterase, Antidiabetic and Anti-quorum Sensing Properties of Bitter Kola (Garcinia kola) and Kolanut (Cola acuminata)

Abstract

Pharmacognosy Research,2023,15,2,373-383.DOI:10.5530/pres.15.2.040Published:February 2023Type:Original Article Authors:Sameh Boudiba, Selcuk Kucukaydin, Alfred Ngenge Tamfu, Kom Blaise, Aristide Mfifen Munvera, Yasmine Arab, Ozgur Ceylan, and Rodica Mihaela Dinica Author(s) affiliations:Sameh Boudiba1, Selcuk Kucukaydin2, Alfred Ngenge Tamfu3,4,*, Kom Blaise5, Aristide Mfifen Munvera6, Yasmine Arab7, Ozgur Ceylan4, Rodica Mihaela Dinica8 1Department of Matter Sciences, Faculty of Exact Sciences, Natural and Life Sciences, Larbi Tebessi University, Tebessa, ALGERIA. 2Department of Medical Services and Techniques, Koycegiz Vocational School of Health Services, Mugla Sitki Kocman University, Mugla, TURKEY. 3Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, Ngaoundere, CAMEROON. 4Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Mugla Sitki Kocman University, Mugla, TURKEY. 5Department of Chemistry, Faculty of Science, University of Ngaoundere, Ngaoundere, CAMEROON. 6Department of Organic Chemistry, Faculty of Science, The University of Yaounde I, Yaounde, CAMEROON. 7Laboratory of Biomolecules and Plant Breeding, Life Science and Nature Department, Faculty of Exact Science and Life Science and Nature, University of Larbi Ben Mhidi Oum El Bouaghi, ALGERIA. 8Dunarea de Jos University, Faculty of Sciences and Environment, Department of Chemistry, Physics and Environment, Galati, ROMANIA. Abstract:Background: Eating Cola acuminata and Garcinia cola nuts in African societies symbolizes in socio-cultural hospitality. They stimulate the nervous system, reduce fatigue and sleep. Objectives: To determine the phenolic composition and bioactivities of G. kola and C. acuminata. Materials and Methods: Hydro-ethanol extracts of the nuts were prepared and their phenolic profiles determined using HPLC-DAD. Antioxidant, anticholinesterase, antidiabetic, antimicrobial, antibiofilm and anti-quorum sensing properties were determined. Results: The most abundant phenolic compound was caffeic acid (105.4±0.75 mg/g) in C. acuminata and myricetin (277.2±0.90 mg/g) in G. kola. The extracts showed good antioxidant activity in five complementary assays and G. kola was more active than both α-tocopherol and BHA standards in the DPPH•, CUPRAC and ABTS•+ assays while C. acuminata was more active than only the α-tocopherol standard in the same assays. Activities were close to those of standards in the β-Carotene-linoleic acid and metal chelation assays. Both extracts had good inhibition of Butyrylcholinesterase (BChE) and Acetylcholinesterase (AChE) with IC50 values 63.27±0.98 μg/mL and 94.15±1.05 μg/mL for C. acuminata and G. kola respectively compared to 5.50±0.25 μg/mL for galantamine in the AChE assay. In the BChE assay, the inhibitory activity was higher for G. kola (IC50 = 38.66±0.80 μg/ mL) that the standard galantamine (IC50 = 42.20±0.48 μg/mL) while that for C. acuminata (IC50 = 87.31±0.77 μg/mL) was moderate. The extracts inhibited α-amylase and α-glucosidase with G. kola (IC50=18.43±0.74 μg/mL) being more active than standard acarbose (IC50=20.52±0.84 μg/mL in the α-glucosidase assay. The nuts could inhibit expression of virulence factors in Chomobacterium violaceum CV12472 by disrupting violacein production and flagellated Pseudomonas aeruginosa PA01 by disrupting swarming motility. Conclusion: The results indicate good nutraceutical potential of both nuts. Keywords:Anticholinesterase, Antidiabetic, Antioxidant, Cola acuminata, Garcinia kola, Quorum-sensing inhibition.View:PDF (380.01 KB)