Abstract
Most of Astragalus genus use for the production of the economically important gum, traganth. The roots of several Astragalus species present a very old and well- known drug in traditional medicine for its usage in the treatment of nephritis, diabetes, leukemia, uterine cancer and as an antiperspirant diuretic and tonic [1]. The purpose of this investigation was the study of the flavonic patterns in 29 plant populations in the west of Iran. Flavonoids were extracted from air-dried leaves, under reflux twice with a Me OH- H2O (7:3) mixture. Pooled extracts of each plant were concentrated at a reduced pressure and the final extract was taken up in a small volume of 80% MeOH [2]. The flavonoids were separated by HPLC using (Auto sampler 360, pump 322, and Diode array detector) and ultra base C- 18 column (5µm, 4.6mm/250mm) [3]. Cluster and discriminate analysis by SPSS (Statistical Package for the Social Sciences) and MVSP (Multivariate Statistical package) with Ward and UPGMA (Unweighted Pair Group Method With Arithmetic Mean) methods showed 7 chemotypes of Astragalus gossypinus in different populations from west of Iran. These chemotypes determined on the base of different quality and quantity of four standards quercetin, flavon, rutin and catechin with other derivatives. A discriminate analysis showed the chemotaxonomic value of the 7 chemical polymorphisms.
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