Abstract
The prevention and treatment of the cancer using plants have attracted increasing interest. The present study was aimed to determine the phenolic compounds of Genista ferox using HPLC-TOF/MS and the anti-oxidant acti-vity associated with anti-cancer activity against human cervical adenocarcinoma (HeLa) cell line. Total anti-oxidant capacities of different extracts of G. ferox were assessed by DPPH assay, and their total phenolic and flavonoids contents measured by FolinCiocalteu and aluminum trichloride assays. The amounts of total phenolic (105.2 ± 0.6 308.5 ± 5.7 mg/g) of extract measured as gallic acid equivalent and flavonoids (8.1 ± 0.1 124.0 ± 0.7 mg/g) of extract measured as quercetin equivalent varied from chloroform to n-butanol extract of the two parts of the plant (leaf and stem). The ethyl acetate extract of G. ferox exhibited the most powerful effect on the DPPH scavenging activity with 94% from the leaf and 93% from the stem, while the chloroform extract from the leaf exhibited the most effective anti-proliferative activity against HeLa cell lines.
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Highlights
Plants used in ancient times as medicines to alleviate symptoms of various ailments (Saeed et al, 2012)
All extracts were subjected to DPPH radical scavenging activity assay to evaluate the anti-oxidant activity as well as the estimation of anti-proliferative activity against human cervical adenocarcinoma (HeLa) cell lines
The total phenolic content was determined by the method of Folin-Ciocalteau using gallic acid as a standard (Singleton and Rossi, 1965). 0.5 mL of gallic acid (1 mg/mL) of different concentrations was mixed with 1 mL of Folin-Ciocalteau reagent (1 N) and allowed to stand at room temperature for 5 min. 5 mL of Na2CO3 (20%) was added
Summary
Plants used in ancient times as medicines to alleviate symptoms of various ailments (Saeed et al, 2012). Many medicinal and aromatic plants contain huge amounts of antioxidants such as polyphenols These substances can have an important role in the absorption and neutralize free radicals, and the extinction of the shirt and triple oxygen or peroxide decomposing. Many of these phytochemicals have anti-oxidant capabilities so they contribute significantly to the fight against many human diseases and contribute to the reduction of mortality (Djeridane et al, 2006). The recent studies on the species of the Genista genus showed pharmacological interest (Belle et al, 1995; Herrera et al, 1992). All extracts were subjected to DPPH radical scavenging activity assay to evaluate the anti-oxidant activity as well as the estimation of anti-proliferative activity against HeLa cell lines
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